RNA-seq of prostate cancer cell lines co-culture with osteoblasts or osteoclasts

Clinically, osteolytic phenotype is rare in prostate cancer. The molecular mechanism of bone metastasis in PCa is not fully understood. We performed RNA-seq to identify osteogenic and tumor associated roles in prostate cancer by a co-culture of osteoblasts (MG63) and prostate cancer cells (C4-2, C4-2B, 22Rv1 and DU145). We compared osteoblastic prostate cancer with osteolytic prostate cancer to evaluate the difference in phenotype of bone metastasis. Osteolytic prostate cancer (DU145 and 22RV1) and osteoblastic prostate cancer (C4-2 and C4-2B) were either mono-cultured or co-cultured with MG63 in horizontal co-culture system (NICO-1). Total RNA was extracted after 5days culture.