Single-cell RNA sequencing analysis of hypopharyngeal carcinoma treated with TPF chemotherapeutic regimen in combination with cinobufotalin treatment

In order to understand the effect of chemotherapy with cinobufotalin combined with TPF on Fadu cell line (hypopharyngeal squamous carcinoma cells), we performed single-cell RNA sequencing on three samples with different treatments. Overall design: Fadu cell line and TPF (paclitaxel, cisplatin and 5-fluorouracil) were used for the construction of TPF-resistant hypopharyngeal squamous carcinoma cell lines. After obtaining a stable TPF-resistant cell line, pre-configured cinobufotalin was added to the culture dishes of Fadu cell line and TPF-resistant cell line, respectively.