Liver regeneration-associated hepatocellular YAP1 activation prevents colorectal cancer liver metastasis through glutamine competition

Literature suggests that hepatocellular Yes-associated Protein 1 (YAP1) signaling is activated following hepatectomy, and that such activation can suppress the growth of metastatic liver tumors. This study aims to explore the mechanisms by which surgical techniques influence colorectal cancer liver metastasis (CRLM). The prognosis of a real-world cohort of 240 CRLM patients undergoing major and minor hepatectomy was compared after adjusting for confounders. To model CRLM, we induced liver metastasis in mice by transsplenically injecting MC38 cells. Cytometry by Time-Of-Flight, RNA sequencing, and untargeted metabolomics were used to explore how surgical choice affected CRLM prognosis. IHC was performed in another real-world cohort of 150 CRLM patients treated with hepatectomy to confirm the role of YAP1 signaling in CRLM progression. We found that CRLM patients and mice undergoing major hepatectomy had longer survival and lower recurrence rates compared to those undergoing minor hepatec..., , # Liver regeneration-associated hepatocellular YAP1 activation prevents colorectal cancer liver metastasis through glutamine competition

Dataset DOI: [10.5061/dryad.rbnzs7hq6 ](10.5061/dryad.rbnzs7hq6)

## Description of the data and file structure

RNA sequencing data included in the manuscript \"Liver regeneration-associated hepatocellular YAP1 activation prevents colorectal cancer liver metastasis through glutamine competition\".

### Files and variables

#### File: Liver_sham_vs_minor_vs_major.csv

**Description:** RNA sequencing data for Figure 2B in the manuscript \"Liver regeneration-associated hepatocellular YAP1 activation prevents colorectal cancer liver metastasis through glutamine competition\".

##### Variables

* Name: Gene symbols
* LR0_1:fpkm: Liver tissue of mice 3 days after sham-operation, replicate 1.
* LR0_2:fpkm:  Liver tissue of mice 3 days after sham-operation, replicate 2.
* LR0_3:fpkm:  Liver tissue of mice 3 days after sham-ope...,