RNA-seq analysis using A549 cells with TRIM22 knockdown and control A549 cells with or without ionizing radiation treatment.

In this study, we explored the role of TRIM22 in the response to ionizing radiation (IR) in A549 human lung cancer cells. TRIM22 was stably knocked down in A549 cells using shRNA, and four experimental groups were established: control A549 cells (non-irradiated), control A549 cells (irradiated), TRIM22 knockdown A549 cells (non-irradiated), and TRIM22 knockdown A549 cells (irradiated). RNA sequencing (RNA-seq) was performed to identify gene expression changes associated with TRIM22 knockdown and irradiation. This dataset provides insights into the molecular mechanisms by which TRIM22 influences the cellular response to radiation and its potential impact on cancer therapy. The experiment was designed to investigate the impact of TRIM22 knockdown on the gene expression profile of A549 cells following ionizing radiation. A549 cells were stably transfected with shRNA targeting TRIM22 or a control shRNA. The experiment included four groups: (1) control A549 cells without irradiation, (2) control A549 cells exposed to irradiation, (3) TRIM22 knockdown A549 cells without irradiation, and (4) TRIM22 knockdown A549 cells exposed to irradiation.Each group included three biological replicates, totaling 12 samples. RNA was extracted from all 12 samples and subjected to RNA sequencing to compare the transcriptomic changes induced by TRIM22 knockdown and ionizing radiation. This design allows for the analysis of the specific role of TRIM22 in the cellular response to radiation.