BIOGRID CURATED DATA FOR PUBLICATION: A genetic interaction map centered on cohesin reveals auxiliary factors involved in sister chromatid cohesion in S. cerevisiae.

Protein-Protein, Genetic, and Chemical Interactions for Ming Sun S (2020):A genetic interaction map centered on cohesin reveals auxiliary factors involved in sister chromatid cohesion in S. cerevisiae. curated by BioGRID (https://thebiogrid.org); ABSTRACT: Eukaryotic chromosomes are replicated in interphase and the two newly duplicated sister chromatids are held together by the cohesin complex and several cohesin auxiliary factors. Sister chromatid cohesion is essential for accurate chromosome segregation during mitosis, yet has also been implicated in other processes, including DNA damage repair, transcription and DNA replication. To assess how cohesin and associated factors functionally interconnect and coordinate with other cellular processes, we systematically mapped the genetic interactions of 17 cohesin genes centered on quantitative growth measurements of >52,000 gene pairs in the budding yeast Saccharomyces cerevisiae Integration of synthetic genetic interactions unveiled a cohesin functional map that constitutes 373 genetic interactions, revealing novel functional connections with post-replication repair, microtubule organization and protein folding. Accordingly, we show that the microtubule-associated protein Irc15 and the prefoldin complex members Gim3, Gim4 and Yke2 are new factors involved in sister chromatid cohesion. Our genetic interaction map thus provides a unique resource for further identification and functional interrogation of cohesin proteins. Since mutations in cohesin proteins have been associated with cohesinopathies and cancer, it may also help in identifying cohesin interactions relevant in disease etiology.

蛋白质-蛋白质、遗传和化学相互作用数据集，由 Ming Sun S (2020) 编著：《以凝聚素为中心的遗传相互作用图谱揭示了参与酿酒酵母S. cerevisiae姐妹染色单体凝聚的辅助因子》：真核生物染色体在间期复制，两条新复制的姐妹染色单体由凝聚素复合物及其辅助因子维系在一起。姐妹染色单体凝聚对于有丝分裂中染色体的准确分离至关重要，同时也与DNA损伤修复、转录和DNA复制等其他过程有关。为了评估凝聚素及其相关因子如何功能性地相互连接并与其他细胞过程协调，我们系统地绘制了以定量生长测量为中心的17个凝聚素基因的遗传相互作用图，涉及超过52,000个基因对的裂殖酵母Saccharomyces cerevisiae。合成遗传相互作用的整合揭示了一个凝聚素功能图谱，包括373个遗传相互作用，揭示了与后复制修复、微管组织和蛋白质折叠的新型功能联系。因此，我们展示了与微管相关的蛋白Irc15以及前折叠蛋白复合物成员Gim3、Gim4和Yke2是参与姐妹染色单体凝聚的新因子。我们的遗传相互作用图谱因此为凝聚素蛋白的进一步鉴定和功能探究提供了一个独特的资源。鉴于凝聚素蛋白突变与凝聚素病和癌症有关，这也有助于识别与疾病病因相关的凝聚素相互作用。curated by BioGRID (https://thebiogrid.org); 摘要：