pSHDY# shuttle plasmid backbone, version w/o mobA Y25F point mutation. Precursor backbone of pSHDY as described by Behle et al., 2019 (https://doi.org/10.1101/757948).
On the sequence of the primers is represented: the restriction site (underlined); the Shine Dalgarno (lower case letter); start or stop codon (bold) for the forward (F symbol in the primer name) or re
