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Richard Allen (University of Hawaii) (2011) CIL:36781, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

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One segment of the assembly line used for phagosome membrane growth in P. caudatum. Cytopharyngeal ribbons bring discoidal vesicles to the left lip of the cytopharynx where the vesicles bind and then fuse with the cytopharyngeal membrane. This expands the membrane to form the phagosome. The role of the contractile cytostomal cord in this process has yet to be determined. Also brought to this area are acidosomes and carrier vesicles. Carrier vesicles contain cargo coming into the cell via parasomal sacs that is sorted from the membrane components that are to be recycled back to the plasma membrane. Sorting occurs in the early endosomes. Coated pits on the early endosomes pinch off to form the carrier vesicles. The carrier vesicles come to lie against the acidosomes where they fuse. Eventually acidosomes will fuse with the phagosome to form the phagoacidosome where the acid pH will kill the bacteria and other food organisms. TEM taken on 5/21/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 15,250X. Bar = 0.25µm.

在刚毛藻(P. caudatum)中,用于吞噬体膜增长的装配线的一段。细胞咽带将碟形囊泡输送到细胞咽的左侧唇部,囊泡在此处结合并与其细胞咽膜融合,从而扩展膜形成吞噬体。此过程中收缩性细胞膜孔带的作用尚未确定。此外,该区域还引入了酸性小泡和载体囊泡。载体囊泡包含通过包囊囊进入细胞的货物,这些货物被从需要回收至质膜的膜成分中分离出来。分离过程发生在早期内体中。早期内体的被覆凹坑收缩形成载体囊泡。载体囊泡到达与酸性小泡相对的位置并与之融合。最终,酸性小泡将与吞噬体融合形成吞噬酸性小泡,其中酸性pH值将杀死细菌和其他食物生物。该透射电子显微镜(TEM)图像由R. Allen于1973年5月21日使用日立HU11A在75kV的条件下拍摄,负片放大倍数为15,250倍。标尺长度为0.25微米。
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