Elucidating the role of RXR in driving the OnF porgram along the CRC malignancy contiuum.

To investigate the role of RXR during eraly OnF reporgramming, we perturbed the APC/RXR regulatory axis during tumor intiation. To investigate the role of RXR in sustaining the OnF state in advanced CRC, we perturbed its expression in AKSP tumoroids. Overall design: We forced RXR expression in APC flox/flox oprganoids, to block its downregulation following APC depletion (tumor intiation), or in advanced CRC tumoroids (AKSP)