Thermus thermophilus TnSeq

<p>In this study we develop a procedure for thermostable selection of random insertion mutants based on a gene cassette encoding a thermostable resistance to kanamycin flanked by the recognition sites (ME sites) for Tn5 transposase. The transposition library generated in vitro was initialy transformed in a PrimPol mutant HB27 derivative (ppol), which shows ~2 log fold transformation efficiency. This allowed the generation of the generation of a large library of insertion mutants (“Mother”), which was later transferred to a wild type HB27 strain (“Daughter”). Both libraries were sequenced straightforward and after several culture passages. See the manuscript for more details.</p>
<p>This dataset contains the raw data of the sequencing libraries as well as the GitHub repository containing the scrips and reports from the analysis.</p>