The regulatory mechanisms of SIS3 on bone marrow-derived NK (BM-NK) cells under TGF-β stimulation using RNA sequencing

To explore the underlying mechanisms of SIS3 attenuating TGF-β-induced immunosuppression on NK cells, RNA-sequencing was performed on primary BM-NK cells under TGF-β stimulation with or without SIS3 pre-treatment. BM-NK cells pre-incubated with 10μM SIS3 for 3 hours were stimulated with 5ng/ml TGF-β1 for another 3 hours. Then cells were harvested as Ctrl group (no stimulation nor SIS3 pre-treatment), T group (with TGF-β simulation but no SIS3 pre-treatment) and SIS group (with TGF-β simulation and SIS3 pre-treatment) and subjected for RNA sequencing.