Bulk RNAseq on WT and TKO E14 PGCLCs

We investigate the impact of DNA methylation on epiblast differentiation. To this end, we used in vitro Epiblast-like cell (EpiLC) differentiation on WT and DNA-methylation free TKO murine ESCs. We next performed bulk RNAseq at D0, D2, D4, D6 and D8 of differentiation to study transcriptomic differences. Comparison of bulk mRNA sequencing results of WT and TKO ESCs (D0), and D2, D4 and D7 cells during PGCLC differentiation