Amplicons for NGS were prepared following cloning of PCR fragments into mammalian expression vectors as outlined in Fig 8A. The average number of full-length reads was determined based on the percenta
The underlined nucleotides indicate overlapping base pairs with the generic primers for the ORFs cloned using the Gateway® system. The bold underlined nucleotides show the restriction site for the app
Massively parallel sequencing is rapidly emerging as an efficient way to quantify biodiversity at all levels, from genetic variation and expression to ecological community assemblage. However, the num
arestriction enzyme sites are underlined in the primer sequences. bto express hexokinase in Pichia pastoris, the forward primer with the BglII site instead of XhoI was used for PCR amplification of ge