Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes of aGVHD mice tranferred Wild Type or OTUD1-/- cells.

Donor OTUD1 deficiency significanltly ameliorates the severity of aGVHD mice. We used single cell RNA sequencing (scRNA-seq) to analyze the immune cells in aGVHD mice transferred wild type or OTUD1-deficient cells. Spleens were isolated from aGVHD mice transferred wild type or OTUD1-deficient cells, then, the spleens were prepared as single cell suspensions and analyzed using scRNAseq.