ChIP-seq of the R-tailocin locus-specific LexA-related regulator PrtR1, two H-NS-like proteins MvaT and MvaV and the cell cycle-associated protein ParB

A ChIP-seq was used to identify the binding regions of the R-tailocin locus-specific LexA-related regulator PrtR1, two H-NS-like proteins MvaT and MvaV and the cell cycle-associated protein ParB in a R-tailocin inducing condition (with mitomycin C) and a non-inducing condition. We used Pseudomonas protegens CHA0 mutants that produce the protein of interest with a C-terminal V5-tag (MvaT-V5, MvaV-V5, PrtR-V5 and ParB-V5). We used CHA0 wild type without tagged proteins as a negative control.