Additional Tables A-D and Figures A-D.

Table A. Linear fitting parameters (R, P) between relative concentrations (N = 35×35) of the cerebral metabolites during embryo development from incubation day 16 to 20 and postnatal day 1. Table B. Relative metabolic levels and statistical analysis. Table C. Area of Nissl-positive neurons within the imaging area (327.7×435.8 μm2) during the embryo incubation. Table D. Number of Nissl-positive neurons (mean ± std, N = 18~31, same for all but only labeled in Tau) within the imaging area (327.7×435.8 μm2) during the embryo development from incubation. Figure A. Sketch of a chick embryo brain in incubation day 18 (dorsal view). All cerebrum tissue samples were dissected from the gray area in this study. Figure B. NMR spectra showed the metabolites of brain tissue degraded during the long experiment time. A, B and C were recorded at 30, 90 and 150 minutes after the tissue exposed at the experimental temperature, respectively. Figure C. Plots represent typical correlations of the relative concentration of mIn and GABA as function of ASC, Gln and 2HBA as function of NAA. The linear fitting (line symbols) and the fitting parameters (R, P) are given in the plots. Figure D. Plots of relative concentration (mean ± std, N = 5) of 12 cerebral metabolites as function of the number of Nissl-positive neurons (mean ± std, N = 5, same for all but only labeled in Tau) within the imaging area (327.7×435.8 μm2) during the embryo development from incubation day 16 to 20 and postnatal day 1 (labeled in mIn). The linear fitting (line symbols) and the fitting parameters (R, P) are given in the plots. (PDF)