Expression data from Triticum aestivum near isogenic lines (TugelaDn0, RWA susceptible; TugelaDn1, RWA resistant; TugelaDn2, RWA resistant; TugelaDn5, RWA resistant) after infestation by Diuraphis noxia biotype SA1. Expression data from Triticum aestivum near isogenic lines (TugelaDn0, RWA susceptible; TugelaDn1, RWA resistant; TugelaDn2, RWA resistant; TugelaDn5, RWA resistant) after infestation by Diuraphis noxia biotype SA1

The wheat Nils expression profiles differ because of their response to aphid feeding (i.e., antibiosis - Dn1; tolerance - Dn2; antixenosis and antibiosis - Dn5) We used GeneChip Wheat Genome Array (Affymetrix, USA) analysis to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process. Overall design: Plants of each cultivar were infested with 10 aphids and incubated for 48 h. All leaves except the first leaf were harvested into liquid N2 and stored at 280°C prior to RNA isolation. Data analysis was conducted using CranBioconductor and using a linear mixed statistical model (Botha et al. 2014, Biology Open (2014) 3, 1116–1126 doi:10.1242/bio.201410280).