A role of the CTCF binding site at enhancer Ea in Tcra rearrangement

We found that the CTCF binding site (named as EACBE) at the enhancer Ea of Tcra is essential for normal Tcra rearrangement.The EACBE deletion impaired the TAD structure of the 3' region of the Tcra/d locus and the interactions between the proximal Va genes and the Ja genes without changing the accessibility of the Ja array. Overall design: Histone modification, repertoire and chromatin conformation profiles of 4-8 weeks old wild type (WT) and EACBE-/- mice were generated by deep sequencing, in duplicate, using Illumina Hiseq.