Transcriptome sequences from the Ischyropsalidoidea genera Sabacon and Acuclavella

This research utiizes a combined phylogenetic approach with transcriptome data in conjunction with Sanger sequence data to investigate a tandem of ancient divergences in the harvestmen superfamily Ischyropsalidoidea (Arachnida, Opiliones, Dyspnoi). We rely on Sanger sequences to resolve nodes within and between closely related genera, and use RNA-seq data from a subset of taxa to resolve a short and ancient internal branch. We use several analytical approaches to explore this succession of ancient diversification events, including concatenated and coalescent-based analyses and maximum likelihood gene trees for each locus. We evaluate the robustness of phylogenetic inferences using a randomized locus sub-sampling approach, and find congruence across these methods despite considerable incongruence across gene trees. Incongruent gene trees are not recovered in frequencies expected from a simple multispecies coalescent model, and we reject incomplete lineage sorting as the sole contributor to gene tree conflict. Using these approaches we attain robust support for higher-level phylogenetic relationships within Ischyropsalidoidea.Preliminary phylogenetic analysis identified a weakly supported topology deep in the ischyropsalidoid species tree. Therefore, we used transcriptome data from ischyropsalidoid exemplars descending from these ancient and rapid diversifications to specifically target these problematic nodes. Some of the transcriptomes have previously been deposited in the NCBI SRA (SRX451776, SRX450937, SRX450964). The data submitted here is from the genera Sabacon and Acuclavella.