Lower limits of detection of MVT and static qPCR.

1 NAE, flat-glass Nucleic Acid Extraction cards (BCSI); Qiagen, Qiagen AllPrep Total Nucleic Acid kit. 2 TSB, trypticase soy broth; 7H9, Middlebrook 7H9 broth with ADC enrichment. All stimulations at 37°C except A. hydrophila, which was stimulated at 30°C. 3 LOD, limit of detection. The LOD of MVT was the lowest density of spiked bacteria to exhibit a positive result, defined as ΔCt > 1 in three out of three replicates. MVT was always negative in the absence of spiked bacteria. 4 LOD, limit of detection. The LOD of qPCR was the lowest density of spiked bacteria to exhibit a positive result, defined as a stronger signal (lower Ct value) than all three no-bacteria controls, in three out of three replicates. 5 Experiment depicted graphically in Figure 1.