Figure S2 Data

Supplementary Figure 2. Dissection of C. albicans infected G. mellonella. There does not appear to be any specific tissue tropism for C. albicans infections in G. mellonella, with visible melanized nodules found in scattered in the Fat Bodies (A-C red arrow), trachea (A,C,D white arrow), and the gut (E,F). Microscopic analysis of the dissected tissue also reveals hyphal structures with reduced pigmentation compared to the yeast-like structures (B). (G) A similar progression of C. albicans morphological progression is seen in dissected tissues infections as is seen during the in vitro microscopy. At 0 minutes, there is no melanin encapsulation of the C. albicans yeast, whereas after 1 h, there is extensive melanization of C. albicans yeast. At 12 h, non-melanized hyphal and pseudohyphal structures are visible along with melanized yeast and potential laterally budding blastoconidium. By 17 h, there appears to be melanized laterally-budded blastoconidium with non-melanized hyphae, similar to what we see between hour 12 and 16 in the timelapse microscopy. Red arrows in (G) indicate yeast, yellow arrows indicate pseudohyphae, green arrows indicate hyphae, and blue arrows indicate potential laterally-budded blastoconidium. Scale bars in (B, D, F) represent 50 µm and in (G) represent 10 µm.