CIL:13887, Saccharomyces cerevisiae. In Cell Image Library
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Cells (MATa tem1::GAL-UPL-TEM1-TRP1 pRS316::eGFP-BFA1–TEM1) were fixed in 2.5% formaldehyde for 10 min, washed twice, and resuspended in 0.1 M potassium phosphate buffer, pH 6.4. Cells were then fixed for 10 min in 80% ethanol and resuspended in 1 mg/ml DAPI. Imaging was performed at 25C using a Leica DM6000 microscope equipped with a 100x/1.40 NA oil immersion objective lens, A4, L5, and TX2 filters, and a digital CCD camera (DFC350, Leica). Pictures were processed with LAS AF (Leica) and ImageJ software.
提供机构：
UC San Diego Library Digital Collections
创建时间：
2021-04-15
