S6 Data -

(A)The amplitude of l-LNv responses to PDF on day 0 in Pdf-GAL4>UAS-Epac1 flies crossed to UAS-RNAi lines of the labeled transcription factors. (B) Trace of PDF responses in Pdf-GAL4>UAS-Epac1 during baseline, in sleep-restricted flies, and Pdf-GAL4>UAS-Epac1 flies expressing UAS-nejRNAi. (C, D) The amplitude of l-LNv responses in Pdf-GAL4>UAS-Epac1/UAS-nejWT and age-matched Pdf-GAL4>UAS-Epac1 flies. (E) The amplitude of l-LNv responses to PDF Pdf-GAL4>UAS-Epac1 flies crossed to UAS-RNAi lines of the listed cell surface receptors. (F) The amplitude of l-LNv responses to PDF in sleep restricted. l-LNv, large ventral lateral neuron; PDF, pigment dispersing factor. (XLSX)