Analysis of replication profile in Cyanobacterium aponinum PCC 10605. Cyanobacterium aponinum PCC 10605

When bacteria with a single chromosome, such as Escherichia coli, grow rapidly in nutrient-rich media, DNA is replicated in a multifork mode, and the oriC/ter ratio becomes >2 yielding a V-shaped profile in the depth of high-throughput sequencing reads (lowest at ter and highest at oriC). For cyanobacteria possessing multiple copies of chromosomes, in which only one or a few copies are replicated from oriC, the oriC/ter ratio approximates 1.0, but still exhibits a slightly V-shaped profile in Synechococcus elongatus PCC 7942. In order to identify the replication origin in Cyanobacterium aponinum PCC 10605, genomic DNA was extracted from the exponentially or stationary phase cultures and analyzed using an Illumina MiSeq System.