Identification of non-coding transcripts regulated by Rap1 and other transcription factors by RNA-seq analysis

Eukaryotic cells utilize several mechanisms to ensure that expression of aberrant non-coding RNAs is limited. Gene looping, chromatin modification or remodeling, and RNA surveillance contribute to ensure the fidelity of transcription and limit non-coding transcripts. Here we identify that in Saccharomyces cerevisiae, the transcription factor Rap1 is critical for limiting the expression of aberrant RNAs, particularly near the highly expressed ribosomal protein genes, and characterize them in the context of other non-coding RNAs regulated by chromatin and transcription related factors.真核细胞借助多种调控机制，以限制异常非编码RNA（non-coding RNAs）的表达。基因环化、染色质修饰与重塑以及RNA监视通路，共同保障转录的保真度，并抑制异常非编码转录本的积累。本研究在酿酒酵母（Saccharomyces cerevisiae）中鉴定发现，转录因子Rap1对限制异常RNA的表达至关重要，尤其在高表达的核糖体蛋白基因的邻近区域；同时，本研究结合其他由染色质及转录相关因子调控的非编码RNA的调控背景，对该调控机制进行了系统表征。