深海来源真菌Talaromyces muroii SCSIO 40439中新生物碱二聚体Dioscysmycine A及Alternariol与衍生物的分离鉴定

采用大米固体培养基发酵培养，萃取后的粗提物使用硅胶柱层析、Sephadex LH-20凝胶柱、半制备HPLC等色谱手段分离纯化化合物，通过HRESIMS、NMR及X-ray等波谱分析以及相关文献比对等方法鉴定结构。使用滤纸片法评价抗菌活性，采用多巴速率氧化法对化合物进行酪氨酸酶抑制活性评价。【结果】分离得到4个化合物，包括1个新颖的生物碱二聚体dioscysmycine A（1）和3个已知化合物alternariol（2）、altenusin（3）、3′-hydroxyalternariol 5-O-methyl ether（4）。化合物1对酪氨酸酶有中等抑制活性，抑制率为58%，化合物2对金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌（MRSA）有一定生长抑制活性。

Fermentation was conducted in rice solid medium. The post-extraction crude extract was subjected to separation and purification of compounds via chromatographic techniques including silica gel column chromatography, Sephadex LH-20 gel column, and semi-preparative HPLC. The structures of the compounds were identified through spectroscopic analyses such as HRESIMS, NMR, and X-ray crystallography, as well as comparison with relevant literature. Antibacterial activity was evaluated using the filter paper disk method, while tyrosinase inhibitory activity of the compounds was assessed by the dopa rate oxidation method.
Results: Four compounds were isolated, comprising one novel alkaloid dimer dioscysmycine A (1) and three known compounds: alternariol (2), altenusin (3), and 3′-hydroxyalternariol 5-O-methyl ether (4). Compound 1 displayed moderate tyrosinase inhibitory activity with an inhibition rate of 58%, whereas compound 2 exhibited certain growth inhibitory activity against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA).

搜集汇总

背景与挑战

背景概述

该数据集聚焦于从深海真菌Talaromyces muroii SCSIO 40439中分离和鉴定新生物碱二聚体Dioscysmycine A及Alternariol衍生物，通过发酵培养、色谱分离和波谱分析，成功获得4个化合物，并发现其具有酪氨酸酶抑制和抗菌活性。数据来源于国家自然科学基金项目，由中国科学院南海海洋研究所的研究团队生产，属于海洋生物学领域。