HibDMPs&EFannotation.xlsx

To determine if hibernation influences DNA methylation independent of age at each of 37,492 CpG sites on a custom Illumina microarray (available from NCBI as platform GPL28271), we fit linear models for DNAm on age using the lm function in R for 15 pairs of <i>E. fuscus</i> samples. We then used the resulting residuals in a linear mixed model to compare winter and summer age-adjusted DNAm measured from the same individual with individual included as a random effect. Significant CpG sites (BY p &lt; 0.05) are referred to as differentially methylated positions (DMPs). Because this analysis compared age-adjusted DNAm in winter to summer, sites with higher DNAm in the winter are referred to as “winter-up” and sites with lower DNAm in the winter are referred to as “winter-down.” We include details from an alignment of the 50 bp microarray probe sequences to the <i>Eptesicus fuscus</i> genome (v.1.0) for 32,217 CpG sites. These sites were categorized as being in either intergenic, 3’ UTR (untranslated region), 5’ UTR, promoter, exon, or intron regions depending on the nearest transcription start site for annotated genes.