Microbial genome collection of aerobic granular sludge cultivated in sequential batch reactor using different carbon source mixtures

Aerobic granular sludge microogranisms are cultivated in a sequential batch reactor (SRB), and a metagenome-assembled genome has been successfully acquired through the use of Illumina short-read and PacBio long-read metagenomics. This genome collection builds on a prior study (PRJEB38840; Aline adler, Sfam 2022) by obtaining raw sequences of short-read and long-read metagenomics. This study utilizes the dataset to construct a hybrid assembly of Illumina short reads (two samples: DNA extraction A and B) and PacBio long reads (one sample: DNA extraction A) using the SPAdes assembler. The resulting metagenomic assemblies are presented for each of the days (71, 322, 427, 740), and samples were fed different carbon source mixtures, including volatile fatty acids (day 71), complex monomeric (day 322 and 427), and complex polymeric (day 740). The study also conducts a comparative analysis of the new and old MAGs and provides the combined dataset to the public (n=759). These MAGs have been dereplicated to obtain representatives (n=233). The whole MAG collection is indexed using the gtdb taxonomy and are accompanied by key metrics such as genome size, number of contigs, and N50 value. Additionally, the study offers R-codes for data analysis.