Expression data from Drd2+ cells of mouse mPFC
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The heterogeneity of cortical dopamine D2 receptor expressing cells is not well characterized We performed microarrays to study expression of all the transcriprts specifically from Drd2+ neurons of mouse mPFC. For this we immunoprecipitated ribosomes from mPFC of D2Cre::RiboTag mouse, where ribosomal subunit Rpl22 is tagged with HA epitope specifically in Drd2+ neurons Mouse mPFC was dissected rapidly on ice and ribosomes were precipitated using anti-HA antibodies. Subsequently RNA was isolated from immunoprecipitated ribosomes using RNA isolation columns.
创建时间：
2018-10-12
