Additional file 8 of Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors

Supplementary file 2. Dose-response experiments for CpomIR41a1 and DsuzIR75d.Stimulus were prepared by diluting compounds in water at different % vol/vol [0.1-3.0] and using 1.0-30 μL aliquots of such dilutions on circle filter paper, preparing a serial set of doses [0.1-90]. Based on respective volumes of neat compound content per stimulus, moles of putrescine, spermidine and hexylamine per doses were calculated based on their densities and molecular weights. Raw data reports ∆spikes/0.5 sec frequencies per dose at each replicate. Data were normalized to the effect of dose 20 for putrescine.Stimuli were prepared by diluting compounds in ethanol at different % vol/vol [0.1-3.0] and using 1.0-20 μL aliquots of such dilutions on circle filter paper, preparing a serial set of doses [0.15-90]. Based on respective volumes of neat compound content per stimulus, moles of hexanoic acid, trimethylamine and butylamine per doses were calculated based on their densities and molecular weights. Raw data reports ∆spikes/0.5 sec frequencies per dose at each replicate. Note: we were not able to establish a precise saturating dose, which for some replicates resulted at DOSE 10 and in others at DOSE 20.