Absolute quantitative metagenomic sequencing

Absolute quantitative metagenomic sequencing enhances conventional metagenomic sequencing by providing absolute copy number detection for genes. This is achieved by adding a specific amount of internal control sequences to sample DNA. These internal control sequences are co-amplified with the sample DNA within the same system to construct the AccuMetaG metagenomic library, followed by sequencing. Internal control sequence data within the sequencing reads are extracted through bioinformatics analysis. A standard curve is then constructed based on their corresponding absolute copy numbers. This standard curve is used to calculate the absolute copy numbers for each contig and gene within the sample. This enables the detection of gene abundance at the absolute copy number level, providing a more accurate reflection of the functional information of microorganisms present in the sample.