Germline NLRP1 mutations cause skin inflammatory and cancer susceptibility syndromes via inflammasome activation
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https://www.ncbi.nlm.nih.gov/sra/SRP082310
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资源简介：
We profiled the transcriptional changes in N/TERT-1 immortalized keratinocytes after doxycylin induction of gain-of-function mutants of the inflammasome sensor protein NLRP1. Overall design: Stable N-TERT/1 cell lines were generated using lentiviral transduction. Experimental group includes doxycline-treated N/TERT cells expressing NLRP1 mutants A54T, M77T and A66V. Control group includes doxyclin-treated vector-transduced N/TERT cells and two NLRP1 mutant-transduced cell lines that were not treated with doxycycline. All cells were harvested three days after induction. Total RNA was isolated using Trizol reagent.
创建时间：
2023-01-11
