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CRISPRi-sequencing S. pneumoniae D39V spv_0476 depletion

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NIAID Data Ecosystem2026-03-12 收录
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CRISPRi-sequencing performed on S. pneumoniae D39V in conditions where spv_0476 is either depleted or complemented, in order to identify which operons become more or less essential when silenced using CRIPRi. Illumina sequencing of the sgRNAs targeting all genetic elements after selection reflects directly the abundance of each sgRNA and thus the essentiality of an operon.

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2021-06-24
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