HUVEC gene expression in response to monocytes. HUVEC gene expression in response to monocytes

Monocytes are central for atherosclerotic vascular inflammation. The human non-classical, patrolling subtype, which expresses high levels of CD16 and fractalkine receptor CX3CR1, strongly associates with cardiovascular events. Compared to classical CD14+ monocytes or transwell cocultures, CD16+ monocytes enhanced endothelial STAT1 and NFκB p65 phosphorylation, upregulated expression of CX3CL1 and IL-1β, numerous CCL and CXCL chemokines and molecules promoting leukocyte patrolling and adhesion such as ICAM1 and VCAM1. Overall design: RNA of HUVECs from two donors was isolated after 24h either direct or transwell coculture with either CD14+ or CD16+ monocytes from two different donors and pooled for gene expression analysis.