Data on the change in the protein profiles of human subcutaneous white adipocytes during differentiation and maturation

We analyzed changes in the protein profiles that occur during differentiation and maturation of cultured human subcutaneous white preadipocytes. We divided the three cell lines (Cell Line-1, Cell Line-2, and Cell Line-3) of Caucasian-derived subcutaneous preadipocytes into five stages: i) subcutaneous preadipocytes as stage-1, ii) after inducing differentiation into adipocytes as stage-2, iii-v) from the initiation of lipid droplet formation to become mature subcutaneous adipocytes was defined as stage-3 to stage-5, depending on lipid droplet amount and formation. Proteins from the cells were extracted at each stage (stage-1 to stage-5), proteolytically cleaved using trypsin, and analyzed using untargeted liquid chromatography and mass spectrometry.