Characterization of in vivo phenotypes on non-treated CRISPitope-engineered HC.PmelKO melanomas in comparison to parental control melanomas by 3'mRNA-Seq

Using a CRISPR-Cas9 based approach we fused a model CD8 T-cell epitope to the C-termini of endogenous gene products. By this approach we could target different endogenous gene products with the same epitope-specific T cell receptor (TCR)-transgenic CD8+ T-cells. Here, we characterized the phenotype of various CRISPitope-engineered melanomas models and parental controls by 3'mRNA-seq. We also analyzed a well-characterized dedifferentiated mouse melanoma for comparison. We validated that all CRISPitope-engineered melanomas models exhibit a differentiated melanoma phenotype.