SP cells from NSCLC cell lines have cancer stem cell-like properties in vivo.

(A) Sorted SP and MP cells from H1650 cell line were implanted into the flanks of SCID mice. Tumor incidence and the average volume (±standard error) of the tumors formed within the indicated time are tabulated. (B) Growth kinetics of tumors from SP and MP cells, measured weekly. The points represent the average (±standard error). (C) Subcutaneous tumors derived from 10,000 H1650-SP cells or 100,000 H1650-MP cells were resected and analyzed for SP phenotype in the presence or absence of FTC. Tumors from SP and MP cells contained predominantly differentiated MP cells.