Photoreceptor loss does not recruit neutrophils despite strong microglial activation

In response to central nervous system (CNS) injury, tissue resident immune cells such as microglia and circulating systemic neutrophils are often first responders. The degree to which these cells interact in response to CNS damage is poorly understood, and even less so, in the neural retina which poses a challenge for high resolution imaging in vivo. In this study, we deploy fluorescence adaptive optics scanning light ophthalmoscopy (AOSLO) to study microglia and neutrophils in mice. We simultaneously track immune cell dynamics using label-free phase-contrast AOSLO at micron-level resolution. Retinal lesions were induced with 488 nm light focused onto photoreceptor (PR) outer segments. These lesions focally ablated PRs, with minimal collateral damage to cells above and below the plane of focus. We used in vivo (AOSLO, SLO and OCT) imaging to reveal the natural history of the microglial and neutrophil response from minutes-to-months after injury. While microglia showed dynamic and progre..., , # Photoreceptor loss does not recruit neutrophils despite strong microglial activation

Dataset DOI: [10.5061/dryad.w3r228143](10.5061/dryad.w3r228143)

## Description of the data and file structure

Data files within are primarily videos and images acquired for a paper \"Photoreceptor loss does not recruit neutrophils despite strong microglial activation\" submitted to eLife.

### Files and variables

#### File: Lesion_paper_data.zip

**Description:** 3 sub-folders contain data acquired using Adaptive optics scanning light ophthalmoscopy (AOSLO or AO for short), a scanning laser ophthalmoscope (Heidelberg Spectralis, or HRA), and ex-vivo confocal microscopy. Within each sub-folder, as follows:  AO*data-->mouse strain and other label-->acquisition modality (confocal or phase-contrast, this folder is not always present)-->mouse # and date of acquisition. exVivo*Confocal*data-->mouse strain and other label for histology-->mouse # and date of acquisition. HRA*data-->mouse # and acquisition d...,