Genome wide mapping of Nr1d1 (Reverb a) binding sites in Th1 (T-helper type 1) cells

The goal of this study was to identify all the sites to which our nuclear receptor Nr1d1 binds to and how this interaction with these various genes is influencing the programming of Th1 cells. Naive T-cells were isolated from C57BL/6 mice and programmed into Th1 cells. The cells were harvested after 3-4 days and to characterize the regions bound by Nr1d1, genome wide ChIP-sequencing using an Nr1d1 antibody was carried out. Short reads from IgG were used as a control for peak detection. Overall design: Examination of Nr1d1 binding sites in genome of programmed Th1 cells.