Friedhoff et al. (2024), Fig 3, FACS data

Fig 3. Lipofectamine transfection of SARS-CoV-2 replicon DNA leads to GFP expression that is dependent on DNA linearization but not T7 RNAP and is remdesivir-insensitive. HEK293T transduced with LPCX(AB) (A) or with LPCX(AB)-T7RNAP (B) were then transfected with pSMART-BAC-T7-scv2 DNA, linearized or not, and in the absence or presence of remdesivir, exactly as in Fig. 2, except that transfections were performed using Lipofectamine 3000. Cells were analyzed by flow cytometry two days later.