de novo transcriptome of the Pacific white snook Centropomus viridis
收藏DataCite Commons2020-08-27 更新2024-07-27 收录
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https://figshare.com/articles/de_novo_transcriptome_of_the_Pacific_white_snook_Centropomus_viridis/7620155/1
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资源简介：
In this dataset, a comprehensive transcriptome was assembled and annotated from separate tissues sampled from one white snook juvenile (<i>Centropomus viridis</i>). Total RNA was isolated from each sample using Zymo total RNA miniprep kit, and multiplexed libraries were constructed from oligo-dT-selected mRNA using the TruSeq Kit (Illumina). An average oif 10 million reads per library were obtained using 2x75 chemistry (Illumina NextSeq 500 platform). Reads were processed with Trimmomatic, and assembled using Trinity ver 2.8.2. Assembled transcripts were conceptually-translated into aminoacid sequences with Transdecoder, using BlastX and HMMER hits against UniProt and pFAM databases for best ORF retention. The assembled transcripts and inferred proteins were annotated by BlastX (evalue < 1e<sup>-5</sup>) and pFAM (UniProt database), and the annotation report was integrated in a TSV database using the pipeline Trinnotate.
提供机构：
figshare
创建时间：
2019-01-23
