The proteins of PK-15 cells infected with porcine parvovirus were identified and quantitatively analyzed by TMT labeling at 6 h and 36 h after infection, and the results were analyzed by gene ontology, KEGG and PPI bioinformatics.

In this project, TMT labeling quantitative proteomics technology was used to carry out the research, and a total of 4769 proteins were identified. The differentially expressed proteins were screened according to the criteria that the expression multiple changed more than 1.5 times (up-regulated more than 1.5 times or down-regulated less than 0.67 times) and P value < 0.05. The differentially expressed proteins were screened according to the criteria that the expression multiple changed more than 1.5 times (up-regulated more than 1.5 times or down-regulated less than 0.67). Among them, taking 6h VS 36h as an example, 120 differentially expressed proteins were up-regulated and 67 down-regulated. Through GO enrichment and KEGG pathway analysis, it was found that these differentially expressed proteins were mainly involved in important biological processes such as regulation of localization, cellular homeostasis, regulation of ion transmembrane transport, cytoskeleton organization and negative regulation of lymphocyte mediated immunity, and mainly involved in the regulation of important KEGG metabolic pathways such as Legionellosis, Steroid biosynthesis, Pentose and glucuronate interconversions, Adherens junction and Endocytosis.