_A_AgroinfGuidedPathwaywithConstitutivedCas9EV-GCMS.output.raw

pISA-tree file path: _p_SUSPHIRE\_I_T12_CuInducible\_S_P1_TransientExp_Nb\_A_AgroinfGuidedPathwaywithConstitutivedCas9EV-GCMS\output\raw Title: Agroinfiltration of guided pathway for moth pheromones production with constitutive dCas9EV system in Nicotiana benthamiana leaves Description: Two constructs were made that comprised the three genes required for moth pheromone production (AtrD11, HarFar and EaDAct/ATF1) under the regulation of the synthetic promoters created in T11. In the constructs it was also included the guideRNA1 and nptII genes for their use in stable transformation. The constructs were first tested transiently in Nicotiana benthamiana leaves via agroinfiltration, together with a plasmid containing the dCas9EV system expressed constitutively or a plasmid containing just P19 as a negative control. Leaf samples were collected after 5 days and the pheromone production was assayed via GC-MS