snRNAseq from sciatic nerves from mouse of 2-3, 15-16, and 20-30 months old

Changes in gene expression during peripheral nerves aging, to the single cell level, are unkown. To explore possible age-related changes during healthy aging of peripheral nerves we compared snRNAseq of sciatic nerves from young adult (2-3 months old), senior adult (15-16 months old), and aged (20-30 month old mice. Overall design: Nerves from WT (C57BL/6) mice were mechanically dissociated and viable (DRAQ7+) nuclei were sorted using Fluorescent activated cell sorting based on size.