Additional file 1 of MicroRNA-214 modulates the senescence of vascular smooth muscle cells in carotid artery stenosis

Additional file 1: Supplemental materials and methods. Figure S1. MicroRNA-214-3p (miR-214) modulates the snenecence of human aortic smooth muscle cells induced by hypoxia. (A) The cellular and exosomal miR-214 in CoC2 treated HASMCs after 48 h (n = 5). (B,D) Representative western blots depicting quaking, TERF1, TERF2, p16INK4, and pRB expression in miR-214 mimic and miR-214 antagomiR transfected HASMCs (n = 3). (C,E) Normalized expressions of quaking, TERF1, TERF2, p16INK4, and pRB (n = 3). (F,H) Senescence-associated β-galactosidase staining demonstrating senescence in miR-214 mimic and miR-214 antagomiR transfected HASMCs. (G,I) Bar graphs show quantification of relative of SA-β-gal positive cells (n = 3). (J,L) Immunofluorescent staining showing the number of Ki-67 positive cells (pink; shown by white arrow) in miR-214 mimic and miR-214 antagomiR transfected HASMCs. (K,M) Bar graphs show quantification of relative of Ki-67 positive cells (n = 3). Bar, 100 μm. Data are presented as the means ± SEM. * P < 0.05; ** P < 0.01 (Two-tailed Student’s t-test).