Transcriptomic analysis by RNA-seq unveils distinct gene expression profile of U397 cells depleted of NSPs

Characterization of transcriptomic changes associated with genetic ablation of three neutrophil serine proteases (CTSG, ELANE, PRTN3). U937 cells depleted of CTSG, ELANE, and PRTN3 simultaneously (?NSPs) were generated using the CRISPR-Cas9 system and sgRNAs. RNA-Seq analysis revealed transcriptomic changes between control cells and ?NSPs, with 170 and 220 genes up- or down-regulated respectively. Overall design: RNA-Seq analysis of three control lines of U937 cells and three independent clones of ?NSPs U937 cells