k562+sequins_dRNA_albacore-2.1.3.tar.gz
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Direct RNA sequencing data for human cell line k562 with ~10% sequins, a spike-in reference standard. <br>Generated on 22 November 2017 using 500ng total RNA extracted from k562 cells and 50ng of RNA sequins Mix A (final concentration after sample preparation = 1.51 ng/uL). Nanopore MinION flowcell FAH35825 (R9.4 chemistry), displayed 1323 active channels at commencement of run. Sequencing protocol RNA001. Base called with Albacore version 2.1.3.Includes albacore summary statistics (bundled in .tgz) and a generic quality report of the run (.pdf). <br>Sequin reference sequences and expected abundances for Mix A can be found at http://www.sequin.xyz/downloads/<br><br><br><br><br>
针对人类K562细胞系的直接RNA测序数据集,该数据集掺入了约10%的sequins(外源 spike-in 参考标准品)。 本数据集于2017年11月22日生成,所用材料为从K562细胞中提取的500ng总RNA与50ng RNA sequins Mix A(样本制备后终浓度为1.51 ng/μL)。测序采用Nanopore MinION测序芯片FAH35825(搭载R9.4化学试剂),测序启动时有效通道数为1323个。本次测序使用RNA001方案,通过Albacore v2.1.3完成碱基识别。数据集包含打包于.tgz压缩包中的Albacore统计摘要,以及本次测序的通用质量报告(.pdf格式)。 Mix A的sequins参考序列与预期丰度信息可从http://www.sequin.xyz/downloads/获取。




