Whole genome mapping of p63 binding sites in ME180 human cervical carcinoma cells

Using tiled microarrays covering the entire human genome, we identify ~5800 target sites for p63, a homolog of the p53 tumor suppressor essential for stratified epithelial development and implicated in epithelial stem cell maintenance. Keywords: ChIP-chip ME180 cells were grown in the absence (-) or presence (+) of the DNA-intercalating agent Actinomycin D (ActD). Crosslinked chromatin from ME180 cells was immunoprecipitated with the 4A4 anti-p63 antibody that recognizes all p63 isoforms.