Effect of senktide administration on the embryo collected from superovulated cows

To clarify the effect of intravenous senktide administration on the quality and developmental stage of collected embryo from superovulated cows, mRNA expression profiles of the embryo were investigated. Hierarchical cluster analysis with the expression levels of all genes was divided these cows into three clusters. First cluster was composed of control cows, second cluster contained senktide (30 nmol/min, 2h) treated cows and third cluster consisted of senktide (300 nmol/min, 2h) treated cows. At 48 h after the start of superovulated treatment, senktide solutions were intravenously infused (30 or 300 nmol/min) for 2 h in cows. All cows were inseminated with frozen–thawed semen 108 and 120 h after the start of superovulated treatment. On 7 days after the first artificial insemination, embryos were collected by nonsurgical uterine flushing. Then, the mRNA expression profiles of the embryo were investigated to clarify the effect of intravenous senktide administration on the quality and developmental stage of collected embryo from superovulated cows.