RNAseq of chicken hepatocellular carcinoma (LMH) cells after 1h and 4h exposure to retinol and retinoic acid

Little focus has been put on elucidating whether RA and RO differ in their effect on gene expression. We conducted an in-depth comparison of the transcriptomic responses to RA (100 nM) and RO (1 uM) in chicken LMH cells. We thereby confirmed that RA exerts a stronger effect on down-stream targets and found only a 76 % overlap in differentially expressed (DE) genes between both treatments. Furthermore, we observed differences in the early response to RA, which indicates an involvement of ncRNAs in the RA response.