M2 Metagenome: Chiken manure and straw compost

Functional metagenomes, by combining new generation sequencing technologies, bio-informatics and genetic engineering, make it possible, even in non-cultivable microbial consortia, to identify enzymes with new technological characteristics, to technologize them and use them in various processes in order to obtain products with "added value".
DNA sample of the first phase compost microflora (based on straw and chicken manure, 75 oC) from Jraber, Armenia (40°20'16.7"N 44°39'19.3"E) was sequenced by long-read nanopore technology using a MinION Mk1B apparatus with R9․4.1 flow cell. A total of 327,921 reads were analyzed with an average of 3269 bases per reads. 98 % Bacteria, 1% Eukaryota and 1% Archaea+Viruses were detected among obtained reads. Representatives of the Deinococcus-Thermus filum were in the lead - 55.75 %, followed by Proteobacteria - 10.44% and Firmicutes - 6.64%. In the genus level Thermus was in majority – 55.66 %, followed by Escherichia – 4.26 % and Pseudomonas – 2.04 %. And in species level Thermus thermophilus composed 49.32 %, followed by Thermus parvatiensis – 4.73 % and Escherichia coli – 4.24 %.
The resulting metagenome contains the necessary number of genes to obtain recombinant cellulases, hemicellulases, and lignin-modifying enzymes for lignocellulose degradation.